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µ-Slide Chemotaxis

µ-Slide Chemotaxis

µ-Slide for stable gradients, 3 chambers on one slide for chemotaxis and migration assays

Instructions (PDF, 1.1 MB)
Coating Procedure (PDF, 59 KB)
Application Note for chemotactical assay (PDF, 2.4 MB)
Chemotaxis and Migration Tool based on ImageJ
Product Information (PDF, 1.5 MB)
Important Information on μ-Slide Chemotaxis (PDF, 549 KB)

Movie: Preparation of µ-Slide Chemotaxis (MV 04)
Movie: Chemotaxis of HT1080 in µ-Slide Chemotaxis (MV 08)
Movie: Chemotaxis of HUVEC blocked by spongistatin (MV 09)


Applications

  • chemotaxis of cancer, endothelial cells, fibroblasts...
  • chemotaxis of slow migrating, adherent cells
  • migration assays with simultaneous cell tracking by (fluorescence) microscopy
 

Technical features

  • 3 chambers on one slide for parallel assays
  • ready to use system, no assembling
  • linear gradients, stable for over 48 hours
  • made for high-end video microscopy



  technical details:
chemotaxis chambers on slide 3
volume per chamber 80 µl
observation area 2x1 mm2
total height with plugs 12 mm
volume chemoattractant 18 µl
bottom matchescoverslip No. 1.5
catalog numbers:
ibiTreat, tissue culture treated / 10 pcs. 80306
coated (Collagen IV) / 10 pcs. 80302
hydrophobic, uncoated / 10 pcs. 80301

µ-Slide Chemotaxis