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Please note that the outer dimensions of the heated stage have a multi-well format. Therefore, it cannot host multi-well plates.
Heating stages
and incubation units
Heating stages for live cell imaging
Instructions (PDF, 2.1 MB)
Add on: Active gas mixer (PDF, 1.3 MB) (Prod. 10920, 10922)
Add on: Passive gas mixer (PDF, 1.2 MB) (Prod. 10921)
Product Flyer (PDF, 1.3 MB)
Product Information (PDF, 3.3 MB)
- universal heating system for all microscope platforms
- micro environment via heated plate and heated transparent lid
- heated transparent lid prevents condensation on µ-Slide lids
- with inserts for µ-Slides, regular slides, or 35 mm dishes
- controller for two separate heating systems
- optional with gas incubation unit
- For catalog numbers please see heating systems overview
Imaging cells in vitro is one of the challenges in today’s microscopy. The two critical parameters that need to be monitored closely are pH and temperature. To monitor and regulate changes in temperature, we developed a heating system that allows for easy temperature control in a very cost effective manner. The heating device can be used with any inverted microscopy system and can be adapted to any microscopy chamber or slide.
This heating stage is a two component system that consists of a heated plate and a heated transparent lid. The base for the heated plate has a multi-well format. In this way, the system can be placed into any regular multi-well frame, delivered with your microscope. So, we have generated a heating device that can be used on microscopes from different manufacturers. It fits into both, manual and automated stages. The heated plate hosts all types of microscopy chambers as µ-Slides and µ-Dishes as well as non ibidi formats.
The heating elements are arranged in such a way that a defined temperature gradient in vertical direction arises. Warming the heated lid to around 40 °C and the heated base plate only to around 37 °C prevents the formation of condensed water at the lid of your microscopy chamber. That in turn, prevents scattering of light or shadow formation and provides a better illumination of the specimen.
 
Controllers for the heating systems
 ibidi offers two different types of controllers to regulate the heat. The HT series comes with a digital heat control. This economic controller is optimized for reliability and is used in cell culture labs world wide. It is used in delicate applications such as live cell imaging and in vitro fertilization. This controller covers 90% of all applications.
In contrast, the ibidi T series heat controllers work with continuous current. Therefore, the T series leads to perfect focus stability and serves extremely high resolution applications at numerical apertures of NA >1.2. The optimization for extremely low focus drift makes the system especially suitable for techniques like TIRF and confocal microscopy. The continuous signal minimizes noise to an extremely low level. That makes these controllers suitable for electronic analysis of cells in patch clamp or impedance measurements.
Gas mixers for the incubation unit
 Additionally, the heated lid comes with a gas inlet. A variety of gas mixers upgrades the system to a complete stage top incubator.
| I) active CO2 mixer |
mixes pressurized air and pure CO2 to various mixtures (1-15% CO2) |
| II) active flexible gas mixer (CO2, N2, O2, air) |
for various gas mixtures; creates regular CO2 and hypoxia conditions |
| III) passive system |
for premixed gases only |
Overview of multi-well format heating system
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