The Extracellular Flux System for
Measuring Oxygen Consumption Rate (OCR)
and Extracellular Acidification Rate (ECAR)

in cooperation with Seahorse Bioscience

Product Flyer (PDF, 2 MB)

Determine cellular bioenergetics in a microplate

The XF Extracellular Flux Analyzer is a fully-integrated instrument that simultaneously measures the two major energy yielding pathways – aerobic respiration and glycolysis. The XF assays provide increased throughput in a drug discovery format. This fast and sensitive measurement of cellular bioenergetics allows to

• study integrated drug delivery for compound or complex experiments
  
• measure Oxygen Consumption Rate (OCR)
  
• determine Extracellular Acidification Rate (ECAR)
  
• measure in a label free and a time-resolved manner
  
• analyze in a microplate-based format (24 well / 96 well) with less variation
  
• reuse the cells after the non-destructive/non-invasive measurement

The Seahorse series comes with 3 versions. All systems determine the Oxygen Consumption Rate (OCR) and the Extracellular Acidification Rate (ECAR). The XF24/3 measures additionally CO₂ production rate (CDPR). For each well there are multiple injection ports so that complex assays or concentration studies can be performed easily.

Measuring Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR)

In this experiment with primary hippocampal neurons the bioenergetic profile was determined, starting with basal respiration. Then ATP turnover and the maximal respiratory capacity was determined.

	OCR	ECAR	CDPR	Injection ports	Working volume
XF24				4	0.5-1.2 ml
XF24/3				4	0.5-1.2 ml
XF96				2	0.2-0.45 ml

The flux packs which are required for the assays consist of a disposable 24-well or 96-well, dual-analyte sensor cartridge, a calibration plate, and a lid. The measured analytes are OCR (oxygen consumption rate) and ECAR (extracellular acidification rate). Two fluorophores for analyte detection are embedded in a polymer spotted on the end of each of the 24 or 96-well sensor sleeves.

Each sensor cartridge also contains a unique multi-well drug delivery system, which injects 25 to 100 µl of up to four different drug compounds into each cell well on command.

For webinars and more information please visit www.seahorsebio.com